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Moisturizing (Recover skin barrier)

Introduction

Moisture retention is said to be the most important in skin care. By doing careful moisture retention, you can keep beautiful clear and glowing skin.
However, as we get older, the skin tends to dry more easily. The dry skin leads to the rough skin, and the stratum corneum of skin that becomes weak by the roughness further loses its moisture. This results in the further progress of skin dryness—you get into a vicious cycle. The surface of rough skin has uneven and rough texture, which could cause skin dullness.
For retaining the skin moisture, it is important to prevent skin roughness by conditioning the stratum corneum of skin carefully to keep the moisture in the inside of skin.
Fullerene has been confirmed to have effects of conditioning the stratum corneum of skin, improving the skin barrier function, and enhancing the moisture retention capacity of skin.

Test of Fullerene’s Moisturizing Effect

Test Methods

The following two kinds of tests were performed.

1) Evaluation of the barrier function recovery rate

The amount of water evaporation from the skin in the inner side of the forearm was measured for each of 10 subjects. After that, the stratum corneum in the inner side of the forearm was exfoliated with the tape stripping method to cause a rough skin, then the amount of water evaporation was measured again. Purified water containing the water-soluble fullerene Radical Sponge at the content of 1% and purified water without fullerene as a control were applied to the specified spots of each subject every morning and every evening (twice a day), and the amount of water evaporation was measured everyday after washing the testing spots to evaluate the barrier function recovery rate.

2) Evaluation of the moisture content of stratum corneum

After doing the tape stripping, test substances were applied to the inner side of the forearm every morning and every evening (twice a day) for 5 days, and the transepidermal water loss (TEWL) and the moisture content of stratum corneum were measured.

Results

(i) The vertical axis shows the barrier function recovery rate, where a larger value means higher recovery effect. Two or three days after the tape stripping, the barrier recovery rate at the water-soluble fullerene Radical Sponge-applied spot significantly increased, compared to the control. This result obviously shows that fullerene recovers the skin barrier function1).

[TRITC staining]
Mature stratum corneum cells・・・・Deep red
They are dyed deeper because they are covered with CE.

Immature stratum corneum cells・・・Light red
They are hard to stain because they are not so much covered with CE.

(ii) "Moist Fullerene" has an effect of retaining the moisture content of stratum corneum even for the skin with reduced barrier function and promotes the recovery of barrier function.

References
1) Murakami, M. et al., Photodermatol. Photoimmunol. Photmed. 29(1), 196-203 (2013).
2) Murakami, M.; Maeda, N., Cosme Tech Japan 3(8), 47-52 (2013).